proteins proteome profiler human cytokine array kit  (R&D Systems)

Journal: Frontiers in Immunology

Article Title: E2F1-induced autocrine IL-6 inflammatory loop mediates cancer-immune crosstalk that predicts T cell phenotype switching and therapeutic responsiveness

doi: 10.3389/fimmu.2024.1470368

Figure Lengend Snippet: The impact of E2F1 on immune cell infiltration and cytokine release in coculture with T cells. (A, B) Correlation of E2F1 expression with infiltration of monocytes, eosinophils, and neutrophils in melanoma patients with primary tumors (A) or metastasis (B) . (C, D) Estimation of CD4 + Th1 and Th2 cell infiltration cells in melanoma patients with primary tumors or metastasis correlated to the expression of E2F1, IL-6 (C) , and STAT3, CD274 (D) . Significant correlations are highlighted in red. (E, F) Cytokine assays of supernatants from different culture/coculture conditions were compared as illustrated showing an E2F1-dependent increased secretion of immunosuppressive type 2 cytokines (E) that was completely reversed after E2F1-KD in melanoma cells (F) . Asterisks indicate transcriptionally upregulated factors. FC, fold change.

Article Snippet: To measure secreted proteins Proteome Profiler Human Cytokine Array Kit was used according to manufacturer’s instruction (#ARY005B, R&D Systems).

Techniques: Expressing

Journal: Frontiers in Immunology

Article Title: E2F1-induced autocrine IL-6 inflammatory loop mediates cancer-immune crosstalk that predicts T cell phenotype switching and therapeutic responsiveness

doi: 10.3389/fimmu.2024.1470368

Figure Lengend Snippet: Mathematical model and overview of the melanoma-CD4 + T cell crosstalk with E2F1-STAT3/IL-6/IL6R axis. (A) Mathematical model of the E2F1-STAT3/IL-6 axis including the auto- and paracrine feedback loops from melanoma and immune cells. The heatmap shows that factors related to the IL6R-STAT3-E2F1 pathway are expressed in melanoma and CD4 + T cells prior to activation and crosstalk. (B) Crosstalk simulation illustrating IL-6 expression in melanoma and CD4 + T cells. (C) Output of simulation representing expression levels of E2F1 or STAT3 that are sufficient to trigger IL-6 secretion in melanoma cells. (D) The impact from E2F1 on the melanoma T cell crosstalk where high E2F1 expression leads to an immunosuppressive TME through feedback loops triggered increased release of IL-6 inducing a Th2 shift (left). In contrast, E2F1 depletion in melanoma cells blocks the augmented IL-6 secretion and, in turn, diminishes type-2 cytokine production favoring an anti-tumor Th1 response.

Article Snippet: To measure secreted proteins Proteome Profiler Human Cytokine Array Kit was used according to manufacturer’s instruction (#ARY005B, R&D Systems).

Techniques: Activation Assay, Expressing

Journal: Nutrients

Article Title: Agarwood Oil Nanoemulsion Attenuates Cigarette Smoke-Induced Inflammation and Oxidative Stress Markers in BCi-NS1.1 Airway Epithelial Cells.

doi: 10.3390/nu15041019

Figure Lengend Snippet: Figure 2. Effect of Agarwood-NE on the CSE-induced transcription of the pro-inflammatory cytokine IL-8. BCi-NS1.1 cells were pre-incubated for 1 h in the presence of 25 and 50 µg/mL agarwood-NE, followed by exposure to 5% CSE for 24 h. The mRNA levels of IL-8 were determined via RT-qPCR. Values are expressed as mean ± SEM (n = 4, *: p < 0.05; ****: p < 0.0001).

Article Snippet: The effects of agarwood-NE on cytokine expression levels in CSE-induced BCiNS1.1 cells were assessed using a human cytokine protein array kit (R&D Systems, Minneapolis, MN, USA), as described in a previous study [12].

Techniques: Incubation, Quantitative RT-PCR

Journal: Nutrients

Article Title: Agarwood Oil Nanoemulsion Attenuates Cigarette Smoke-Induced Inflammation and Oxidative Stress Markers in BCi-NS1.1 Airway Epithelial Cells.

doi: 10.3390/nu15041019

Figure Lengend Snippet: Figure 3. Effect of Agarwood-NE on the CSE-induced production of pro-inflammatory mediators in human cytokine protein array. BCi-NS1.1 cells were pre-incubated for 1 h in the presence of 25 and 50 µg/mL agarwood-NE, followed by exposure to 5% CSE for 24 h. The protein levels of IL-1α (A), IL-1β (B), IL-1Ra (C), and GDF-15 (D) were determined via human cytokine protein array.

Article Snippet: The effects of agarwood-NE on cytokine expression levels in CSE-induced BCiNS1.1 cells were assessed using a human cytokine protein array kit (R&D Systems, Minneapolis, MN, USA), as described in a previous study [12].

Techniques: Protein Array, Incubation